From a therapeutic perspective, this enables the one molecule to be. Application published us200604209 summary nanoparticle technologies provide novel means to deliver diagnostic and therapeutic agents across cellular barriers. Fattigationplatform nanoparticles using apotransferrin. Turbidity and particles in the samples can interfere with the assay. Sulphonated nps are relatively hydrophobic so the protein binding is expected to be stronger than for carboxylic ones. Examples of nano excipients apo transferrin nanoparticles golla et al. Chiral surface of nanoparticles determines the orientation. Transferrin has several advantages for targeted delivery to cancer cells. Fattigationplatform nanoparticles using apotransferrin stearic acid as a core for receptor oriented cancer targeting. Nanoparticles have been proposed as carriers for drugs, genes and therapies to treat various diseases1, 2. Limited success of antisense oligonucleotides aso in clinical anticancer therapy calls for more effective delivery carriers. Transferrin iron starvation therapy for lethal bacterial. Maghemite nanoparticles stabilize the protein corona formed with.
Compared with albuminbased nanoparticles, our transferrin nanoparticles showed more effective targeting ability. Human transferrin has been biolabelled with gold nanoclusters au ncs using a simple, fast and nontoxic method. Maghemite nanoparticles stabilize the protein corona. Transferrin is synthesized as a 698 amino acid aa precursor that is divided into a 19 aa signal sequence plus a 679. An efficient targeted drug delivery through apotransferrin. Selfassembled ir780loaded transferrin nanoparticles as. Apotransferrin definition of apotransferrin by medical. Study of the bionano interface in human plasma plos one, dec 2019 andrzej s. A polyethyleniminecontaining and transferrinconjugated. Cyclodextrin nanosponge encapsulated with camptothecin improved delivery of camptothecin to prostate minelli et al.
Transferrinfunctionalized nanoparticles lose their. Irondeficient, apo transferrin is commonly used to supplement culture media that are formulated with elevated levels of. Study of the bionano interface in human plasma article pdf available in plos one 77. Transferrin, bovine apo form, lyophilized catalog number. Transcytosis and brain uptake of transferrincontaining. Apotransferrin plasma, blood, and related proteins and. Selfassembled targeted nanoparticles based on transferrin. It is a bilobed molecule that is the product of an ancient gene duplication event 1, 6. The cellular uptake of nanoparticles was higher compared to free paclitaxel in transferrin receptoroverexpressing breast cancer cell lines. Inhibition was seen at twenty microgramsml in culture medium. Transferrin is responsible for 50% to 70% of the iron binding capacity of serum. The nanoparticleprotein interaction and the protein corona. An efficient targeted drug delivery through apotransferrin loaded.
Transferrin has a molecular weight of around 80 kda. For the determination a bradford protein assay with pure holotransferrin as standard was applied for the determination of the average amount of. An efficient targeted drug delivery through apotransferrin loaded nanoparticles. The challenge of effectively delivering therapeutic agents to brain has led to an entire field of active research devoted to overcome the blood brain barrier bbb and efficiently deliver drugs to brain. Apotransferrin has been shown to strongly inhibit bacterial adhesion to biomaterials including polystyrene, polymethylmethacrylate, and silicone. Transferrin glycoproteins bind iron tightly, but reversibly. Introduction transferrin is the irontransport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, cd71. Apoferritin definition of apoferritin by medical dictionary. Transferrin has two ironbinding sites and is largely, but not exclusively, synthesized by the liver. Increased brain uptake of targeted nanoparticles by adding. Spions using one selected corona protein, transferrin, was. Monitoring characteristics and genotoxic effects of engineered.
Curcumincontaining apotransferrin nanoparticles were pre pared using soloil. The role of cellpenetrating peptide and transferrin on. A similar compound produced in the intestinal mucosa is apoferritin. It is a glycoprotein, but also known more specifically, as an ironstorage. Transferrinconjugated polymeric nanoparticle for receptor. Download fulltext pdf download fulltext pdf transferrin coated nanoparticles. Packaging 1, 5 g in poly bottle 100, 500 mg in poly bottle other notes nonheme irontransport protein. Morphology, size distribution, fourier transform infrared ftir spectra, encapsulation efficiency, and loading capacity of tfhacmcplga nps were characterized. Apotransferrin nanoparticles were developed by soloil chemistry. Transferrin, protein beta1 globulin in blood plasma that transports iron from the tissues and bloodstream to the bone marrow, where it is reused in the formation of hemoglobin. Apo transferrin is not bound to iron molecules but willing and ready to do so in the cell culture dish, and if your cultures have serum in it, there will be plenty of fe to go around. Serotransferrin, transferrin, siderophilin, beta1metalbinding globulin, tf, pro1557, pro2086, dkfzp781d0156, apo transferrin, atf. Human transferrin, apo, iron free and holo iron saturated.
The tumorspecific ligands of polymeric nanoparticles trigger. Here, we report on the interaction of superparamagnetic maghemite. Biocompatibility, absorption and safety of protein nanoparticle. Thus, stearic acid modified apotf was used as primary biocompatible material of a nanoparticlebased drug delivery system to target cells that overexpress the tfr on their surface. Purification of transferrin from cohn supernatant i using. Intravenous transferrin, rgd peptide and dualtargeted. Human transferrin is offered as a lyophilized powder in both apo ironfree and holo ironsaturated forms.
Instead of using apotf as a np ligand, apotf was made amphiphilic by conjugating with stearic acid to form selfassembled nps in water. Monopoli1,2, francesca baldelli bombelli1,3, kenneth a. Fattigationplatform nanoparticles using apotransferrin stearic acid as a core for receptor oriented cancer targeting hardik h. These nanocrystals transferrin conjugated nanoparticles technology fields. Tumortargeting transferrin nanoparticles for systemic. Transcytosis and brain uptake of transferrin containing nanoparticles by tuning avidity to transferrin receptor devin t. Contaminant human transferrin assay molecular devices. Thus, tf sorting may be able to occur more normally, whereas the rest of the nanoparticle remains a passenger in the transcytotic vesicle. Size and surface functionalization of iron oxide nanoparticles. Synthesis and characterization of human transferrin.
It is a glycoprotein, but also known more specifically, as an ironstorage protein, found in. Transferrin conjugated particles can cross the bloodbrain barrier and target rat brain tumor glioma cells in in vitro models pulkkinen et al. However, the extent of delivery for even nanoparticles can be limiting for. Tdds is a transferrinmodified polyd,llactidecoglycolide plga and carboxymethyl chitosan cmc nanoparticle loaded with a photosensitizer hypocrellin a ha, named tfhacmcplga nps. Serum transferrin facilitates the transport and uptake of iron cells in culture.
Pdf an efficient targeted drug delivery through apotransferrin. Therefore, particulate matter should be removed by centrifugation prior to running the assay. Transferrin tf is widely used as a tumortargeting ligand for the delivery of anticancer drugs because the tf receptor is overexpressed on the surface of various fastgrowing cancer cells. Transferrintailored solid lipid nanoparticles as vectors. Holo transferrin, is already bound to iron, and is used in cultures with low levels of iron as a supplement. The molar ratio autf 120 was found to be the optimal value to promote the formation of a high concentration of gold clusters in transferrin. Study of the bionano interface in human plasma andrzej s. Fetal bovine serum fbs was an effective dispersing agent for tio2 nanoparticles in all six media due to synergistic effects of its multiple protein components, successfully reproduced using a simple fbs mimic protein cocktail containing similar concentrations of bsa. The platform was constructed based on the hypothesis that a combination of. The synthesized nanoparticle tflpng39 contained a small molecular weight. Atf protein human apo transferrin natural protein prospec. The goal of this study was to develop a nanoparticle system for delivery of aso g39, which targets mrna of antiapoptotic protein bcl2, to acute myeloid leukemia aml cells. In this article, we report on tf nanoparticles as an sirna delivery carrier for in vivo tumorspecific gene silencing.
In the past few decades, transferrin mediated drug delivery has been widely used for targeting delivery of drugs to tumor 43. Solid lipid nanoparticles for targeted brain drug delivery. Dawson1,2 1centre for bionano interactions, school of chemistry and chemical biology, university college dublin, dublin, ireland, 2conway institute for biomolecular and. Found fixed to the surface of developing red blood cells, transferrin frees iron directly into the cell. A conjugation of stearic acid to apotransferrin, fattigationplatform, as. Transferrin is another abundant protein in the blood. Coating nanoparticles with plantproduced transferrin hydrophobin fusion protein enhances their uptake in cancer cells article pdf available in bioconjugate chemistry 286. Human transferrin is an 80,000 dalton glycoprotein found in human serum that facilitates transport of iron between cells. Transferrin levels rise with iron deficiency and fall in cases of iron overload. Human transferrin tf is a single chain, 80 kda member of the anionbinding superfamily of proteins 1 5. Many strategies have been developed to target nanomaterials to specific or overexpressed receptors in diseased cells, and these typically involve functionalizing the surface of nanoparticles with proteins, antibodies or other biomolecules. Fe 2 o 3 nanoparticles with human blood plasma protein transferrin presenting different ironsaturation levels. Transferrin is widely used as a tumortargeting ligand in cancer treatment and diagnosis since the transferrin receptor is overexpressed on the cell membrane of various types of cancer cells.115 988 1202 1347 1466 77 544 1118 1238 1541 398 1566 1567 440 43 1057 505 459 63 379 842 961 721 1297 1331 1375 886 305 527 893 259 753 356 916 1283